15 research outputs found
Two dimensional angular domain optical imaging in biological tissues
Optical imaging is a modality that can detect optical contrast within a biological sample that is not detectable with other conventional imaging techniques. Optical trans-illumination images of tissue samples are degraded by optical scatter. Angular Domain Imaging (ADI) is an optical imaging technique that filters scattered photons based on the trajectory of the photons. Previous angular filters were limited to one dimensional arrays, greatly limiting the imaging capability of the system.
We have developed a 2D Angular Filter Array (AFA) that is capable of acquiring two dimensional projection images of a sample. The AFA was constructed using rapid prototyping techniques. The contrast and the resolution of the AFA was evaluated. The results suggest that a 2D AFA can be used to acquire two dimensional projection images of a sample with a reduced acquisition time compared to a scanning 1D AFA
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Origin, migration and fate of newly generated neurons in the adult rodent piriform cortex.
Newly generated neurons are continuously added to the olfactory epithelium and olfactory bulbs of adult mammals. Studies also report newly generated neurons in the piriform cortex, the primary cortical projection site of the olfactory bulbs. The current study used BrdU-injection paradigms, and in vivo and in vitro DiI tracing methods to address three fundamental issues of these cells: their origin, migratory route and fate. The results show that 1 day after a BrdU-injection, BrdU/DCX double-labeled cells appear deep to the ventricular subependyma, within the white matter. Such cells appear further ventral and caudal in the ensuing days, first appearing in the rostral piriform cortex of mice at 2 days after the BrdU-injection, and at 4 days in the rat. In the caudal piriform cortex, BrdU/DCX labeled cells first appear at 4 days after the injection in mice and 7 days in rats. The time it takes for these cells to appear in the piriform cortex and the temporal distribution pattern suggest that they migrate from outside this region. DiI tracing methods confirmed a migratory route to the piriform cortex from the ventricular subependyma. The presence of BrdU/NeuN labeled cells as early as 7 days after a BrdU injection in mice and 10 days in the rat and lasting as long as 41 days indicates that some of these cells have extended survival durations in the adult piriform cortex